Course description
Are you unsure of your microscopy skills?
The LCI microscopy course will provide you with all the theoretical and practical knowledge that you need to improve your microscopy experiments.
- Can your sample preparation be improved?
- Which resolution does your experiment require?
- How to choose the pixel size on a confocal?
- Which objective is most appropriate for your own experiment?
- How to extract meaningful information from your images?
- How to easily build publication-grade figures?
The course is fully online, yet highly hands-on as you will do all the exercises using your own sample and your own microscope.
Note:
- Students must prepare their own sample before the course starts and need to plan accordingly.
- The course is intensive and requires to be fully committed during the course period, as well as during a few days of work (including preparing samples) throughout January, before the course starts. Do not plan any heavy experiments or meetings during the course period.
- Researchers who have not yet used microscopy or do not have an active microscopy project are advised to get trained at their local imaging facility, actively acquire images for at least 3 months then apply to the LCI course next year.
Please read the course syllabus and what is written behind the + sign above before sending your application.
Prerequisites and Selection
Prerequisite courses, or equivalent
In their application, applicants must confirm that they fulfil the following criteria:
a) have an active microscopy project involving imaging of a fluorescent sample, started minimum 3 months prior to the start of the course;
b) be able to prepare their sample and bring/ship it to the course;
c) In their lab or local facility, have been trained on and have regularly used a fluorescence microscope able to acquire images of fluorescent samples;
d) have access to that microscope during the course (some assignments require submitting new images);
e) set aside time so they can be fully committed to the 4 full-week equivalent course (total 160h): a few days preparation throughout January (preparing their samples, downloading software, etc), 10 full days of synchronous work (2-3 days/week, total 80h) + 2-3 days/week of self-paced work.
Researchers who have not yet used microscopy or do not have an active microscopy project are advised to get trained at their local imaging facility, actively acquire images for at least 3 months then apply to the LCI course next year.
Selection
Applications will only be considered if they contain ALL 4 parts listed below.
- The confirmation that the applicants fulfil the a, b, c, d and e Prerequisites which are listed above (and in the syllabus);
- A brief description of the scientific aim of their own imaging projects. If you have several projects, describe only the most relevant and prepare the corresponding sample for the course;
- A brief description of their microscopy experience so far, when they started their current microscopy project and which microscope(s) they currently use;
- Fluorescent images of their sample acquired by them on the microscope mentioned above and submitted in the original format delivered by the microscope (please write to Sylvie.le.guyader@ki.se to ask for a link to upload your original images).
The selection will be based on:
- The relevance of the course syllabus for the applicant’s individual study plan/research (according to written motivation),
- The applicants' recent microscopy experience, and thus the applicants’ potential to follow the course and fulfil the imaging assignments,
- The start date of doctoral studies (priority given to earlier start date).
Course director
Sylvie Le Guyader
Link to website
https://ki.se/en/research/research-infrastructure-and-environments/core-facilities-for-research/live-cell-imaging-core-facility-lci/lci-microscopy-course
Course syllabus
H7F2870
Department
Department of Medicine, Huddinge
Doctoral programme
Development and Regeneration (DevReg)
Type of course
**Other course
Keywords
Microscopy