Course description
This course will doctoral students and postdocs to acquire the necessary knowledge to address experimentally keypoints of metabolic characterization of experimental models in diabetes research.
The course is laboratory based, aiming to give all participants hands on experience with isolation of pancreatic islets, skeletal muscle and adipose tissue. Techniques for measurement of glucose transport in isolated rodent skeletal muscle, of lipolysis in isolated adipocytes, and for studying insulin release from pancreatic islets will be covered.
Theoretical and practical considerations will be presented and discussed.
After the course the students will be able;
1) to measure glucose transport in isolated rodent skeletal muscle
2) to measure lipolysis in isolated adipocytes
3) to dissect out mouse pancreatic islets and measure the insulin release
4) to judge and analyze obtained data.
The students will also be able to describe the possibilities and limitations of the above techniques.
Requirements and Selection
Prerequisite courses, or equivalent
No prerequisite courses, or equivalent, demanded for this course.
Selection
Selection will be based on:
1) the relevance of the course syllabus for the applicant’s doctoral project (according to written motivation).
2) start date of doctoral studies (priority given to earlier start date).
Course director
Alexander Chibalin
Course syllabus
K1F3121
Department
Department of Molecular Medicine and Surgery
Doctoral programme
Metabolism and Endocrinology (MetEndo)
Type of course
**Other course
Keywords
endocrinology, metabolism, laboratory technique iabetes