Course description
The purpose of this course is to give the students an in-depth theoretical understanding of the technologies used for generating precise genetic modifications in mice. The course focuses on the classic gene targeting technique (homologous recombination in embryonic stem cells), CRISPR/Cas9 (non-homologous end joining and homology directed repair), and transgenesis by pronuclear injection in zygotes (e.g. TCR transgenes). Understanding these technologies will enable the students to design experiments to test hypotheses in vivo and generate new tools to ask complex immunological questions. These skills are becoming ever more important as science is getting more complex.
Requirements and Selection
Prerequisite courses, or equivalent
Swedish law and ethics on the protection of laboratory animals (theory).
Selection
Selection will be based on 1) the relevance of the course syllabus for the applicant's doctoral project (according to written motivation), 2) date for registration as a doctoral student (priority given to earlier registration date)
Course director
Alexander Espinosa
Course syllabus
K2F2186
Department
Department of Medicine, Solna
Doctoral programme
Allergy, immunology and inflammation (Aii)
Type of course
**Other course
Keywords
Gene targeting, Transgenesis, CRISPR/Cas, Mouse models, Immunology